منابع مشابه
Tbx5 is dispensable for forelimb outgrowth.
Tbx5 is essential for initiation of the forelimb, and its deletion in mice results in the failure of forelimb formation. Misexpression of dominant-negative forms of Tbx5 results in limb truncations, suggesting Tbx5 is also required for forelimb outgrowth. Here we show that Tbx5 is expressed throughout the limb mesenchyme in progenitors of cartilage, tendon and muscle. Using a tamoxifeninducible...
متن کامل03-P088 Regulation of Tbx5 protein function during forelimb development
with this construct to obtain a permanent clone cell line in which the Fgf8b-EGFP could be secreted on the extracellular matrix. The inductive activity of Fgf8b-EGFP protein was proved either in vitro (cell cultures) as in ex-vivo (organotypic explant cultures of E9.5 mouse neural tube). Ectopically, Fgf8b-EGFP induces the phosphorilation of the final effector of the FGF signalling cascade (ERK...
متن کاملTbx5 Buffers Inherent Left/Right Asymmetry Ensuring Symmetric Forelimb Formation
The forelimbs and hindlimbs of vertebrates are bilaterally symmetric. The mechanisms that ensure symmetric limb formation are unknown but they can be disrupted in disease. In Holt-Oram Syndrome (HOS), caused by mutations in TBX5, affected individuals have left-biased upper/forelimb defects. We demonstrate a role for the transcription factor Tbx5 in ensuring the symmetric formation of the left a...
متن کاملTbx5 is essential for heart development.
Mutations in the Tbx5 transcription factor cause heart septal defects found in human Holt-Oram Syndrome. The complete extent to which Tbx5 functions in heart development, however, has not been established. Here we show that, in Xenopus embryos, Tbx5 is expressed in the early heart field, posterior to the cardiac homeobox transcription factor, Nkx2.5. During morphogenesis, Tbx5 is expressed thro...
متن کاملThe limb identity gene Tbx5 promotes limb initiation by interacting with Wnt2b and Fgf10.
A major gap in our knowledge of development is how the growth and identity of tissues and organs are linked during embryogenesis. The vertebrate limb is one of the best models to study these processes. Combining mutant analyses with gain- and loss-of-function approaches in zebrafish and chick embryos, we show that Tbx5, in addition to its role governing forelimb identity, is both necessary and ...
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ژورنال
عنوان ژورنال: Development
سال: 2007
ISSN: 1477-9129,0950-1991
DOI: 10.1242/dev.02622